How to judge the target protein in gel filtration, molecular sieve chromatography


Release time:

2023-04-19

Source:

network

Molecular sieve gel filtration separates different substances by molecular size, and is often used for desalting protein solutions; for protein solutions with determined molecular weights, the elution time of the target protein can be estimated by comparing the molecular weights. The properties of various proteins can only be identified by following each protein peak. How should the elution time of the target protein be estimated by comparing the molecular weight?

Molecular sieve gel filtration separates different substances by molecular size, and is often used for desalting protein solutions; for protein solutions with determined molecular weights, the elution time of the target protein can be estimated by comparing the molecular weights. The properties of various proteins can only be identified by following each protein peak. How should the elution time of the target protein be estimated by comparing the molecular weight?


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How to judge the target protein in gel filtration, molecular sieve chromatography

Adsorption principle of a molecular sieve

l cations (such as Na+, K+, Ca2+, Li+, etc.) exist in the crystal lattice to balance the excess negative charge in the crystal. According to the crystal structure, molecular sievesare mainly divided into: A type, X type, Y type, etc.

Increase efforts to reduce emissions in various places, eliminate backward production capacity

September 17,Hangzhou -- According to the “News and Newspapers Abstract”, China National Radio (CNR), local governments have further increased energy conservation and emission reduction efforts. Several small thermal power units were dismantled by blasting in Zhejiang; in Jiangsu,176 companies that produce, sell and use Ozone Depleting Substance (ODS) have completed the elimination or replacement work.

Properties and applications of molecular sieves

The function of a molecular sieve in adsorption or repulsion is affected by the electrical properties of the molecule. Synthetic zeolites have a special function of selective adsorption depending on the size and polarity of the molecules, so that the gas or liquid can be dried or purified, which is the basis for the separation provided by the molecular sieves. Synthetic zeolites can meet a wide range of industrial requirements for adsorption and selective properties, and synthesize zeolite molecular sieves are also widely used in industrial separations.

A synthetic hydrated aluminosilicate (zeolite) or natural zeolite

A synthetic hydrated aluminosilicate (zeolite) or natural zeolite with the function of screening molecules. Its general chemical formula is (M'2M)O·Al2O3·xSiO2·yH2O, and M', M are monovalent and divalent cations such as K+, Na+, Ca22+, Ba22+, etc. respectively. It has many channels with uniform pore size and neatly arranged pores in its structure. Molecular sieves with different pore sizes separate molecules of different sizes and shapes. According to the different molecular ratios of SiO2 and Al2O3, molecular sieves with different pore sizes are obtained. Its models are: 3A (potassium A type), 4A (sodium A type),

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